Parallel Image Analysis of Morphological Yeast Cells*
نویسندگان
چکیده
Fermentation is a critical process for the production simpler substances from organic molecules or could be used to obtain the ethanol by the anaerobic breaking down of sugar. In this work we present different image analysis steps in order to characterize the cell morphology during the biotechnology process. The cell morphology is an important element of the stress which could disturb the production of the biomass or a metabolite. For this purpose we develop a Java software dedicated to an automatic analysis. The software allows us to have information about the growth cells, morphometric analysis (volume/surface) and morphology (budding cells). We want to have the information in biological real time to be able to modify the control parameters, therefore the image is cut into small slices which are analyzed separately by a parallel algorithm.
منابع مشابه
SCMD: Saccharomyces cerevisiae Morphological Database
To study the global regulation of cell morphology, a number of groups have recently reported genome-wide screening data for yeast mutants with abnormal morphology. Despite the relatively simple ellipsoidal shape of yeast cells, in the past, cell morphology researchers have processed information on cells manually. These time-consuming, entirely subjective tasks motivated us to develop image-proc...
متن کاملMeasurement of Morphological Characteristics of Raw Cane Sugar Crystals Using Digital Image Analysis
Raw cane sugar is one of the most important product in the sugar industry and is the main raw material for the white sugar production. Morphological and physical properties of this product might influence the final white sugar. For instance, the behavior during centrifugation, transport and storage is related to the characteristics of these crystals. The object of this study was to determine th...
متن کاملDigital Image Analysis of Yeast Single Cells Growing in Two Different Oxygen Concentrations to Analyze the Population Growth and to Assist Individual-Based Modeling
Nowadays control of the growth of Saccharomyces to obtain biomass or cellular wall components is crucial for specific industrial applications. The general aim of this contribution is to deal with experimental data obtained from yeast cells and from yeast cultures to attempt the integration of the two levels of information, individual and population, to progress in the control of yeast biotechno...
متن کاملQuantitative analysis of colony morphology in yeast.
Microorganisms often form multicellular structures such as biofilms and structured colonies that can influence the organism's virulence, drug resistance, and adherence to medical devices. Phenotypic classification of these structures has traditionally relied on qualitative scoring systems that limit detailed phenotypic comparisons between strains. Automated imaging and quantitative analysis hav...
متن کاملHigh-resolution Live Cell Imaging of the Yeast Life Cycle
Layout, operation, and results of a microfluidic device, which allows for anaerobic and aerobic cultivation of yeast cells over extended time (>3 days) are presented. The use of this device in combination with automated image analysis software and multiple fluorescence reporters (>4 fluorescence protein per strain) allows for studying timing and coherence of morphological features, the cell-cyc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2006